: Using flow cytometry to track how immune cells "wake up" when they detect a threat. Quick Tips for Researchers Temperature Matters : Loading the dye at room temperature ( ) often works better than 37∘C37 raised to the composed with power C
: Always keep your Fura-2 samples in the dark (wrapped in foil) as much as possible, as the dye will "bleach" and stop working if exposed to too much light. Te-AИ™ Fura
Guidelines for the use of flow cytometry and cell sorting ... - PMC : Using flow cytometry to track how immune
: The "AM" stands for acetoxymethyl ester . This chemical modification makes the dye membrane-permeant, allowing it to easily slip through the fatty outer layer of a cell. - PMC : The "AM" stands for acetoxymethyl ester
: Once inside the cell, natural enzymes called esterases chop off the AM group. This "traps" the dye inside the cell and makes it sensitive to calcium.
Fura-2AM is a that allows scientists to see and measure changes in intracellular calcium concentrations (