Imaging Flow Cytometry: Methods And Protocols -

: Non-specific binding is prevented using anti-Fc antibodies. Cells are then stained with fluorophore-conjugated antibodies, viability dyes, or nuclear dyes like DAPI or Hoechst to provide spatial landmarks.

: Systems like the ImageStream use Charge-Coupled Device (CCD) cameras with Time Delay Integration (TDI) to track cells in flow, preventing motion blur and enabling the detection of dim markers. Imaging Flow Cytometry: Methods and Protocols

: Beyond fluorescence, IFC provides data on cell size, shape, circularity, and internal complexity. : Non-specific binding is prevented using anti-Fc antibodies

: If internal markers (e.g., nuclear proteins) are the target, cells are fixed with formaldehyde and permeabilized with detergents like Triton X-100. IFC provides data on cell size