The exterior of the bone or tooth is usually mechanically removed (sanding) or treated with bleach and UV light to remove surface contaminants. 3. Extraction Methods
Once extracted, the DNA must be prepared for Next-Generation Sequencing (NGS). Ancient DNA: Methods and Protocols
All work must be done in a dedicated "Clean Lab" with HEPA filtration, positive air pressure, and UV sterilization. Researchers wear full-body suits to prevent shedding their own DNA onto the samples. The exterior of the bone or tooth is
The goal of extraction is to release DNA from the mineral matrix (bone) while removing inhibitors like humic acids. positive air pressure