Alpha-naphthol Test -

This reaction produces a characteristic purple or violet-colored ring at the interface (junction) between the acid and the test solution. Laboratory Procedure The test is typically performed following these steps: biochemical and: OneLook Thesaurus

The reaction depends on the ability of strong mineral acids to carbohydrates. Dehydration: When concentrated sulfuric acid ( H2SO4cap H sub 2 cap S cap O sub 4 alpha-naphthol test

) is added to the sample, it dehydrates the carbohydrates into aldehyde derivatives. (5-carbon sugars) form furfural . Hexoses (6-carbon sugars) form 5-hydroxymethylfurfural . (5-carbon sugars) form furfural

These furfural derivatives then react (condense) with alpha-naphthol (the Molisch reagent). The , more commonly known as the Molisch

The , more commonly known as the Molisch test , is a highly sensitive qualitative chemical assay used to detect the presence of carbohydrates in a given sample. Named after the Austrian botanist Hans Molisch , it serves as a preliminary screening tool to differentiate carbohydrates from other biomolecules like proteins and lipids. Principle of the Test