: Bifunctional agents can link two DNA bases together (interstrand or intrastand cross-linking), which prevents the DNA strands from separating for replication.
The primary goal of these agents is to disrupt the DNA replication process. They achieve this through a process called : alkalating agent
: Repair enzymes in the cell may attempt to fix the damaged DNA, which often results in further strand breaks and fragmentation. : Bifunctional agents can link two DNA bases
: Once the DNA damage becomes too severe to repair, the cell's internal checkpoints trigger programmed cell death (apoptosis). From old alkylating agents to new minor groove binders alkalating agent